Evaluation of the Cytocompatibility of Fluoride Varnish and Its Effect on Human Gingival Fibroblasts (hGFs): An In Vitro Study

Introduction Untreated dental decay poses a significant oral health challenge, leading to pain, tooth loss, and infections. Fluoride varnishes are in prolonged contact with the surface this prevents decay. However, limited research has been conducted regarding cytotoxicity cytocompatibility of on cells. Recent studies have shed light cytotoxic effect these human fibroblast Material Methods The fibroblasts were isolated cultured 0.00001, 0.0001, 0.001, 0.01, 0.1, 1 % fluoride concentration cells incubated for 72 hours at temperature 37°C cell viability after application varnish was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay. Results This study observed that had dependant gingival (hGFs). As increased, decreased. At 1% concentration, there maximum cytotoxicity. lowest (0.00001), more than 78% found be viable. Conclusion Further is necessary develop safer biocompatible formulations ensure their efficacy preventing caries without causing harm tissues.